7M14
x-ray structure of cj1430 in the presence of GDP, a GDP-D-glycero-4-keto-D-lyxo-heptose-3,5-epimerase from campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-29 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 134.286, 83.709, 110.044 |
Unit cell angles | 90.00, 94.74, 90.00 |
Refinement procedure
Resolution | 27.830 - 2.100 |
R-factor | 0.2049 |
Rwork | 0.202 |
R-free | 0.26380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ryk |
RMSD bond length | 0.007 |
RMSD bond angle | 1.595 |
Data reduction software | PROTEUM PLUS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 69127 | 8583 |
<I/σ(I)> | 9.7 | 2.4 |
Completeness [%] | 97.3 | 93 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 22-27% PEG-5000, 100 mM MOPS, 10 mM GDP |