7M13
Crystal structure of CJ1428, a GDP-D-GLYCERO-L-GLUCO-HEPTOSE SYNTHASE from campylobacter jejuni in the presence of NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9878 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.365, 131.326, 58.855 |
| Unit cell angles | 90.00, 105.76, 90.00 |
Refinement procedure
| Resolution | 30.950 - 1.500 |
| R-factor | 0.1637 |
| Rwork | 0.163 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.641 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 129208 | 6167 |
| <I/σ(I)> | 35.5 | 3.8 |
| Completeness [%] | 96.9 | 92.9 |
| Redundancy | 3.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 16-20% PEG-5000, 150 mM MgCL2, 100 mM MOPS |
