7M07

Pre-catalytic ternary complex of DNA Polymerase Lambda with bound 1-nt gapped SSB substrate and incoming dUMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	56.003, 59.665, 139.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.360 - 1.570
R-factor	0.1713
Rwork	0.171
R-free	0.18770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pfo
RMSD bond length	0.010
RMSD bond angle	0.986
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.570	1.570
Number of reflections	65998	3193
<I/σ(I)>	26.8	1.75
Completeness [%]	99.9	99.1
Redundancy	6.5	4.5
CC(1/2)	0.990	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	37.5mM Na cacodylate pH 6.5, 150mM KCl, 75mM magnesium acetate, 7.5% (w/v) PEG 8000