7LZ2
Structure of glutamate receptor-like channel GLR3.4 ligand-binding domain in complex with methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 74.581, 74.581, 508.887 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.960 - 1.500 |
| R-factor | 0.1859 |
| Rwork | 0.185 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7lz0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.878 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 127.220 | 127.220 | 1.530 |
| High resolution limit [Å] | 1.500 | 8.220 | 1.500 |
| Rmerge | 0.062 | 0.033 | 1.180 |
| Rmeas | 0.064 | 0.035 | 1.226 |
| Rpim | 0.018 | 0.010 | 0.330 |
| Total number of observations | 1796831 | 11236 | 89595 |
| Number of reflections | 136052 | 1087 | 6600 |
| <I/σ(I)> | 19.3 | 55.2 | 1.9 |
| Completeness [%] | 99.9 | 99.1 | 100 |
| Redundancy | 13.2 | 10.3 | 13.6 |
| CC(1/2) | 0.999 | 0.999 | 0.654 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 2 M ammonium sulfate |
