7LZ1
Structure of glutamate receptor-like channel GLR3.4 ligand-binding domain in complex with serine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 74.509, 74.509, 507.975 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.610 - 1.510 |
| R-factor | 0.2038 |
| Rwork | 0.202 |
| R-free | 0.23230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7lz0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.718 |
| Data reduction software | DENZO |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 72.570 | 72.570 | 1.540 |
| High resolution limit [Å] | 1.510 | 8.270 | 1.510 |
| Rmerge | 0.086 | 0.038 | 1.486 |
| Rmeas | 0.089 | 0.040 | 1.541 |
| Rpim | 0.023 | 0.011 | 0.404 |
| Total number of observations | 2020815 | 13282 | 92162 |
| Number of reflections | 133111 | 1070 | 6458 |
| <I/σ(I)> | 15.4 | 43.3 | 1.5 |
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 15.2 | 12.4 | 14.3 |
| CC(1/2) | 0.999 | 0.998 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 2 M ammonium sulfate |
