7LZ0
Structure of glutamate receptor-like channel GLR3.4 ligand-binding domain in complex with glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 74.337, 74.337, 513.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.460 - 2.290 |
| R-factor | 0.2112 |
| Rwork | 0.209 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vea |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.251 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 64.460 | 64.380 | 2.370 |
| High resolution limit [Å] | 2.290 | 8.880 | 2.290 |
| Rmerge | 0.123 | 0.028 | 0.809 |
| Rmeas | 0.130 | 0.030 | 0.856 |
| Rpim | 0.042 | 0.011 | 0.275 |
| Total number of observations | 6217 | 33632 | |
| Number of reflections | 39519 | 885 | 3657 |
| <I/σ(I)> | 12.9 | 31.2 | 2.8 |
| Completeness [%] | 99.7 | 99.6 | 97 |
| Redundancy | 9.3 | 7 | 9.2 |
| CC(1/2) | 0.998 | 0.999 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 277 | 2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6 |
