7LYG

Zika virus RNA promoter stem-loop A with tRNA-scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1271
Spacegroup name	C 2 2 2
Unit cell lengths	157.073, 156.588, 72.325
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.036 - 3.804
R-factor	0.2315
Rwork	0.227
R-free	0.27150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lyf
RMSD bond length	0.003
RMSD bond angle	0.678
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.890
High resolution limit [Å]	3.800	3.800
Number of reflections	6864	6864
<I/σ(I)>	23.4
Completeness [%]	95.9
Redundancy	10.4
CC(1/2)	0.998	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	100 mM Tris pH 8.1, 24% (w/v) polyethylene glycol 3350, 200 mM LiSO4, and 67 mM CsCl