7LX9
T4 lysozyme mutant L99A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.82647 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.246, 60.246, 96.317 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.175 - 1.190 |
| R-factor | 0.2152 |
| Rwork | 0.215 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w57 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.772 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 52.175 | 52.170 | 1.210 |
| High resolution limit [Å] | 1.190 | 6.520 | 1.190 |
| Rmerge | 0.120 | 0.033 | 5.329 |
| Rmeas | 0.124 | 0.034 | 5.515 |
| Rpim | 0.028 | 0.008 | 1.375 |
| Total number of observations | 8891 | 46613 | |
| Number of reflections | 65444 | 480 | 3121 |
| <I/σ(I)> | 16.1 | 90.8 | 0.6 |
| Completeness [%] | 99.9 | 100 | 97.3 |
| Redundancy | 19.5 | 18.5 | 14.9 |
| CC(1/2) | 1.000 | 0.999 | 0.229 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | Isopropanol, PEG 4000, Tris-Cl pH 8.0, Beta-mercaptoethanol, 2-hyrdoxyethyl disulfide |
