7LWR
Structural and Biochemical Insight into Assembly of Molecular Motors Involved in Viral DNA Packaging
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-20 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.721, 49.507, 74.472 |
| Unit cell angles | 82.12, 86.58, 67.37 |
Refinement procedure
| Resolution | 21.690 - 2.350 |
| R-factor | 0.1485 |
| Rwork | 0.147 |
| R-free | 0.16460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7lw0 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.741 |
| Data reduction software | APEX 2 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.700 | 3.500 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 21002 | 19898 |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 99.8 | |
| Redundancy | 2.68 | |
| CC(1/2) | 0.987 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 2.5 M ammonium sulfate, 5% isopropanol |
