7LW0
Structural and Biochemical Insight into Assembly of Molecular Motors Involved in Viral DNA Packaging
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.748, 42.772, 57.222 |
| Unit cell angles | 89.98, 89.98, 89.88 |
Refinement procedure
| Resolution | 34.260 - 2.320 |
| R-factor | 0.1222 |
| Rwork | 0.119 |
| R-free | 0.15170 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.581 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Number of reflections | 16731 | 1681 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 97.5 | |
| Redundancy | 2.6 | |
| CC(1/2) | 0.960 | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | isopropanol 5%, 10mM magnesium acetate, tris pH 7.0 |
