7LVU

Structure of RSV F-directed VHH Cl184

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9774
Spacegroup name	P 43 2 2
Unit cell lengths	73.600, 73.600, 221.979
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.600 - 1.940
R-factor	0.2088
Rwork	0.207
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vak
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.900	1.990
High resolution limit [Å]	1.940	1.940
Number of reflections	46193	2987
<I/σ(I)>	17.1
Completeness [%]	99.9
Redundancy	10.9
CC(1/2)	0.998	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES pH 7.5, 2.0 M ammonium formate