7LVP

Cryptococcus neoformans AIR synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	85.300, 85.769, 37.018
Unit cell angles	90.00, 91.70, 90.00

Refinement procedure

Resolution	42.880 - 2.240
R-factor	0.1983
Rwork	0.197
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vk4
RMSD bond length	0.002
RMSD bond angle	0.421
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.880	2.310
High resolution limit [Å]	2.240	2.240
Rmerge	0.078	0.693
Rmeas	0.092	0.823
Number of reflections	12876	1148
<I/σ(I)>	0.148	0.026
Completeness [%]	99.7	96.8
Redundancy	6.9	6.6
CC(1/2)	0.999	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M SPG (succinic acid, sodium dihydrogen phosphate, and glycine in ratio of 2:7:7), 25% w/v PEG1500