7LVO

Cryptococcus neoformans GAR synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	87.104, 76.489, 74.968
Unit cell angles	90.00, 107.57, 90.00

Refinement procedure

Resolution	38.240 - 2.000
R-factor	0.246
Rwork	0.242
R-free	0.28550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qk4
RMSD bond length	0.004
RMSD bond angle	0.506
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.520	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.050	0.349
Rmeas	0.067	0.479
Number of reflections	31460	7982
<I/σ(I)>	12.4	2.7
Completeness [%]	99.0	99.6
Redundancy	3.4	3.4
CC(1/2)	0.997	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1 M sodium citrate, pH 5.0, 30% v/v MPEG550