7LVM
CASP8 isoform B DED domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.418, 50.414, 52.609 |
| Unit cell angles | 90.00, 116.64, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.470 |
| R-factor | 0.2009 |
| Rwork | 0.199 |
| R-free | 0.23250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Internal structure |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.297 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.030 | 1.550 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.044 | 0.414 |
| Number of reflections | 37049 | 5704 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 96.6 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 100mM Hepes pH 7.5, 20% Ethylene Glycol, 18% PEG 3350, 200mM Sodium Chloride |
