7LV7
Crystal Structure of Branched-chain amino acid aminotransferase from Giardia lamblia ATCC 50803
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-14 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.990, 243.180, 60.500 |
Unit cell angles | 90.00, 118.92, 90.00 |
Refinement procedure
Resolution | 47.530 - 2.100 |
R-factor | 0.1617 |
Rwork | 0.161 |
R-free | 0.19940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dqn as per Morda |
RMSD bond length | 0.007 |
RMSD bond angle | 0.863 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.150 |
High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
Rmerge | 0.095 | 0.032 | 0.433 |
Rmeas | 0.116 | 0.039 | 0.544 |
Number of reflections | 85876 | 946 | 6254 |
<I/σ(I)> | 9.45 | 28.1 | 2.58 |
Completeness [%] | 99.0 | 93.8 | 98.1 |
Redundancy | 2.951 | 2.921 | 2.545 |
CC(1/2) | 0.994 | 0.997 | 0.707 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 287 | RigakuReagents JCSG+ screen, condition H3: 100mM BisTris pH 5.5, 25% (w/V) PEG 3350: GilaA.10478.a.A1.PW27569 + 2mM PLP: tray 254466 h3: cryo: 20% ethylene glcol: puck bfw3-4 |