7LV2

GSQASS segment from the Nucleoprotein of SARS-CoV-2, residues 179-184

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	4.770, 13.600, 42.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.951 - 1.301
Rwork	0.249
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ideal beta strand AAAAAA
RMSD bond length	0.017
RMSD bond angle	1.751
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	XSCALE (Jan 31, 2020)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	12.951	12.951	1.390
High resolution limit [Å]	1.300	3.680	1.300
Rmerge	0.126	0.051	1.045
Rmeas	0.161	0.066	1.306
Number of reflections	809	45	139
<I/σ(I)>	3.47	9.62	0.69
Completeness [%]	93.2	76.3	95.9
Redundancy	2.266	2	2.432
CC(1/2)	0.997	0.998	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	sodium potassium tartrate, lithium sulfate, TRIS, pH 7.0