7LUZ

GQTVTK segment from the Nucleoprotein of SARS-CoV-2, residues 243-248

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	19.570, 4.780, 22.930
Unit cell angles	90.00, 94.00, 90.00

Refinement procedure

Resolution	22.874 - 1.101
Rwork	0.124
R-free	0.14970
Structure solution method	AB INITIO PHASING
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	XSCALE (Jan 31, 2020)
Phasing software	SHELXD (2013/2)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.874	22.874	1.170
High resolution limit [Å]	1.100	2.620	1.100
Rmerge	0.085	0.048	0.446
Rmeas	0.105	0.059	0.607
Number of reflections	1726	166	170
<I/σ(I)>	6.02	15.21	1.37
Completeness [%]	87.1	95.4	50.9
Redundancy	2.71	2.5	2.024
CC(1/2)	0.995	0.993	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	2M Ammonium Sulfate, HEPES, pH 7.5, PEG 400