7LUG
Crystal structure of the pnRFP B30Y mutant
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03321 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.284, 34.107, 89.222 |
Unit cell angles | 90.00, 110.79, 90.00 |
Refinement procedure
Resolution | 41.850 - 1.950 |
R-factor | 0.1897 |
Rwork | 0.189 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lqo |
RMSD bond length | 0.005 |
RMSD bond angle | 1.531 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.850 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 17539 | 1245 |
<I/σ(I)> | 10.8 | |
Completeness [%] | 99.3 | |
Redundancy | 3.56 | |
CC(1/2) | 0.998 | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 286 | Sodium phosphate, citric acid, PEG400 |