7LU3
X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.88557 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.732, 67.732, 101.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.870 - 1.160 |
R-factor | 0.1424 |
Rwork | 0.141 |
R-free | 0.17210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ltd |
RMSD bond length | 0.007 |
RMSD bond angle | 0.890 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.860 | 1.180 |
High resolution limit [Å] | 1.160 | 1.160 |
Rmerge | 0.061 | 2.815 |
Rpim | 0.022 | 1.033 |
Number of reflections | 82307 | 3943 |
<I/σ(I)> | 12.7 | 0.7 |
Completeness [%] | 99.9 | 98 |
Redundancy | 8.5 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (0.5 M Sodium Nitrate). |