7LTU

AALALL SEGMENT FROM THE NUCLEOPROTEIN OF SARS-COV-2, RESIDUES 217-222, CRYSTAL FORM 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	9.450, 11.340, 20.270
Unit cell angles	74.88, 79.13, 67.77

Refinement procedure

Resolution	19.469 - 1.122
Rwork	0.155
R-free	0.19530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ideal beta strand AAAAAA
RMSD bond length	0.010
RMSD bond angle	1.306
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.470	19.470	1.180
High resolution limit [Å]	1.120	3.550	1.120
Rmerge	0.084	0.074	0.397
Rmeas	0.107	0.094	0.511
Number of reflections	2270	85	136
<I/σ(I)>	5.92	11.51	2.02
Completeness [%]	78.4	95.5	31.1
Redundancy	2.366	2.341	2.375
CC(1/2)	0.985	0.982	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	4	298	trifluoroacetic acid