7LTQ
Crystal Structure of a histidine kinase from Burkholderia ambifaria MC40-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9774 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.870, 95.870, 205.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.900 - 2.150 |
R-factor | 0.1701 |
Rwork | 0.168 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MR-rosetta starting from pdb entry 6NB0 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.797 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MR-Rosetta |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.210 |
High resolution limit [Å] | 2.150 | 9.620 | 2.150 |
Rmerge | 0.073 | 0.026 | 0.588 |
Rmeas | 0.079 | 0.028 | 0.639 |
Number of reflections | 60058 | 673 | 4403 |
<I/σ(I)> | 19.11 | 57.4 | 3.01 |
Completeness [%] | 99.6 | 84.9 | 99.8 |
Redundancy | 6.904 | 6.155 | 6.658 |
CC(1/2) | 0.999 | 0.999 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 287 | Hampton Research Crystal Screen HT condition G1: 1.8M ammonium sulfate, 100mM MES monhydrate pH 6.5, 10mM Cobalt (II) chloride: BuamA.00863.e.A1.PS01103 at 28.3mg/ml: tray 223322 g1: cryo: likely 25% EG: puck aog4-2. |