7LT9

Crystal structure of Ras suppressor-1 in complex with PINCH-1 LIM4-5 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-29
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97919
Spacegroup name	I 2 2 2
Unit cell lengths	51.042, 144.384, 185.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.571 - 3.050
R-factor	0.201444108787
Rwork	0.200
R-free	0.23435
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lt8
RMSD bond length	0.015
RMSD bond angle	1.166
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.050	3.050
Rmerge	0.091	0.852
Number of reflections	13446	669
<I/σ(I)>	27.76225
Completeness [%]	97.1	99.7
Redundancy	4.6
CC(1/2)	0.992	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	296	PEG8000