7LT6
Structure of Partial Beta-Hairpin LIR from FNIP2 Bound to GABARAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.910, 44.750, 78.480 |
| Unit cell angles | 90.00, 118.54, 90.00 |
Refinement procedure
| Resolution | 39.460 - 1.800 |
| R-factor | 0.2309 |
| Rwork | 0.229 |
| R-free | 0.27340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hyo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.687 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.460 | 39.430 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
| Rmerge | 0.230 | 0.075 | 0.716 |
| Rmeas | 0.302 | 0.094 | 0.886 |
| Rpim | 0.193 | 0.056 | 0.516 |
| Total number of observations | 951 | 6695 | |
| Number of reflections | 42191 | 332 | 2519 |
| <I/σ(I)> | 10.7 | 26.3 | 2 |
| Completeness [%] | 97.5 | 88.8 | 98.4 |
| Redundancy | 2.8 | 2.9 | 2.7 |
| CC(1/2) | 0.748 | 0.958 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.1 M magnesium acetate, 0.1 M MOPS pH 7.5, and 12% v/v PEG 8000 |
