7LST
Ruminococcus bromii Amy12-D392A with 63-a-D-glucosyl-maltotriosyl-maltotriose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2016-10-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.981, 99.107, 167.236 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 85.260 - 2.050 |
R-factor | 0.1824 |
Rwork | 0.180 |
R-free | 0.23180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lsa |
RMSD bond length | 0.004 |
RMSD bond angle | 0.877 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.300 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 49996 | 4926 |
<I/σ(I)> | 5.9 | |
Completeness [%] | 99.8 | |
Redundancy | 7.3 | |
CC(1/2) | 0.990 | 0.640 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 8.75 - 8.9 mg/ml of protein and 10 mM 63-a-D-glucosyl-maltotriosyl-maltotriose via hanging drop against a well solution containing 16% PEG 3350, 4% glycerol, 0.3 ammonium acetate, and 0.1 M Bis-Tris pH 6.5. |