7LSQ
The Crystal Structure of Q108K:K40E:T53A:R58W:Q38F:Q4F:Y19W Mutant of HCRBPII Bound with LizFluor Chromophore Showing Excited State Intermolecular Proton Transfer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-22 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.766, 54.990, 69.805 |
| Unit cell angles | 109.34, 97.80, 102.60 |
Refinement procedure
| Resolution | 34.954 - 1.671 |
| R-factor | 0.186257687006 |
| Rwork | 0.185 |
| R-free | 0.23188 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rct |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.053 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.954 | 1.731 |
| High resolution limit [Å] | 1.671 | 1.671 |
| Rmerge | 0.044 | 0.518 |
| Number of reflections | 54659 | 5195 |
| <I/σ(I)> | 24.7 | |
| Completeness [%] | 96.4 | 91.1 |
| Redundancy | 2.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | Ammonium acetate, PEG 4000, Sodium acetate |
