7LSB
Structure of B1, a monoclonal VEGFR2 antibody parental of KD035
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 63.15 |
| Detector technology | CCD |
| Collection date | 2016-02-02 |
| Detector | PHILLIPS |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.691, 66.416, 66.543 |
| Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
| Resolution | 42.125 - 2.492 |
| R-factor | 0.1912 |
| Rwork | 0.184 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2n9g |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.125 | 2.580 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 16360 | 1520 |
| <I/σ(I)> | 23.61 | |
| Completeness [%] | 97.7 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.900 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.2M Na Phosphate monobasic monohydrate, 20% PEG3350 |
