7LRU

Crystal structure of SFPQ-NONO-SFPQ chimeric protein homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95373
Spacegroup name	C 2 2 21
Unit cell lengths	73.665, 107.162, 68.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.420 - 1.600
R-factor	0.2204
Rwork	0.218
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wii
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.380	45.380	1.630
High resolution limit [Å]	1.600	8.760	1.600
Rmerge	0.053	0.033	2.110
Rmeas	0.055	0.035	2.243
Rpim	0.015	0.010	0.746
Total number of observations	468399	2907	15470
Number of reflections	36031	255	1786
<I/σ(I)>	19.4	63	1
Completeness [%]	100.0	98.1	100
Redundancy	13	11.4	8.7
CC(1/2)	1.000	0.999	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15%(v/v) Jeffamine ED-2003, 10% EtOH