7LR7

Crystal structure of GH5_18 from Streptomyces cattleya in complex with GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	I 2 2 2
Unit cell lengths	47.151, 115.761, 159.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.330 - 1.950
R-factor	0.1723
Rwork	0.170
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lqx
RMSD bond length	0.007
RMSD bond angle	0.843
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.790	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	32258	2207
<I/σ(I)>	8.4
Completeness [%]	99.7
Redundancy	12
CC(1/2)	0.994	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	15% PEG 3350, 0.2 M sodium thiocyanate