7LR1

Crystal structure of GH5_18 from Bifidobacterium longum subsp. longum ATCC 55813

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-20
Detector	RAYONIX MX300HE
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	105.420, 143.730, 155.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.480 - 1.800
R-factor	0.1405
Rwork	0.139
R-free	0.17260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lqx
RMSD bond length	0.020
RMSD bond angle	1.496
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.610	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	218071	10715
<I/σ(I)>	8.6
Completeness [%]	99.9
Redundancy	5
CC(1/2)	0.995	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.22 M ammonium phosphate monobasic