7LQX
Crystal structure of a GH5_18 from Bifidobacterium longum subsp. infantis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97911 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.830, 51.720, 85.450 |
| Unit cell angles | 90.00, 91.24, 90.00 |
Refinement procedure
| Resolution | 44.400 - 1.300 |
| R-factor | 0.1366 |
| Rwork | 0.136 |
| R-free | 0.15180 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.903 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.400 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 95210 | 4688 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 7.5 | 7.3 |
| CC(1/2) | 0.999 | 0.908 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 8 % PEG 2000, 0.2 M lithium sulfate, 0.1 M Bis-Tris pH 6.0 |
