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7LQ0

Crystal structures and ribonuclease activity of the Flavivirus host factor ERI3 that is involved in viral RNA synthesis define the ERI subfamily of structure-specific 3-prime - 5-prime exoribonucleases

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-2
Synchrotron siteSSRL
BeamlineBL9-2
Temperature [K]100
Detector technologyPIXEL
Collection date2021-01-11
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.97946
Spacegroup nameP 21 21 21
Unit cell lengths58.529, 74.200, 96.487
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution37.230 - 1.600
R-factor0.1636
Rwork0.162
R-free0.19920
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2xri
RMSD bond length0.012
RMSD bond angle1.124
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwarePHENIX (1.18.2_3874)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]37.2301.700
High resolution limit [Å]1.6001.600
Number of reflections36898860361
<I/σ(I)>23.15
Completeness [%]99.8
Redundancy6.59
CC(1/2)1.0000.636
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP29037.5% PEG 3350, 20% saturated KCl, 200 mM Imidazole / Malate buffer 8.0, 18mM AMP, 3.3 mM N-{2-[4-(thiophen-2-yl)-1H-imidazol-2-yl]ethyl}-2,3-dihydro-1,4-benzodioxine-2-carboxamide (from Life Chemicals)

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PDB entries from 2024-05-15

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