7LPG

APE1 product complex with abasic ribonucleotide DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-14
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	44.118, 61.077, 73.039
Unit cell angles	83.68, 78.07, 87.49

Refinement procedure

Resolution	23.690 - 2.080
R-factor	0.2214
Rwork	0.219
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dff
RMSD bond length	0.009
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.140
High resolution limit [Å]	2.080	2.080
Rmeas	0.119
Number of reflections	75243	2151
<I/σ(I)>	12.5
Completeness [%]	99.5	97.9
Redundancy	3.6	3.3
CC(1/2)		0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	200 mM MgCl2, 100 mM Sodium Citrate (pH 5), 10-15% PEG 20,000