7LP3

Structure of Nedd4L WW3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 2 21 21
Unit cell lengths	24.565, 55.522, 82.583
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.040 - 1.610
R-factor	0.1811
Rwork	0.178
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2mpt
RMSD bond length	0.010
RMSD bond angle	1.116
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.040	1.575
High resolution limit [Å]	1.610	1.520
Rmerge	0.040	0.614
Rmeas	0.056	0.869
Number of reflections	18091	1756
<I/σ(I)>	7.05	1.5
Completeness [%]	99.2	96.03
Redundancy	2	2
CC(1/2)	1.000	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		310	1.6M (NH4)2SO4, 0.1M Potassium Sodium Tartrate, 5% glycerol, 0.1M Sodium Acetate