7LOY
Dihydrodipicolinate synthase with pyruvate from Candidatus Liberibacter solanacearum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2018-12-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953723 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.777, 133.581, 155.988 |
| Unit cell angles | 90.00, 100.55, 90.00 |
Refinement procedure
| Resolution | 46.270 - 2.400 |
| R-factor | 0.20313 |
| Rwork | 0.201 |
| R-free | 0.25191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i7u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.428 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.320 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 79992 | 79992 |
| <I/σ(I)> | 1.04 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4 | |
| CC(1/2) | 0.917 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% w/v PEG3000, 0.1 M sodium citrate, pH 5.5 |
