7LO2
S-adenosylmethionine synthetase cocrystallized with CTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.139, 118.320, 144.456 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.220 - 1.890 |
| R-factor | 0.1674 |
| Rwork | 0.166 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pl7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.041 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.440 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 137355 | 6706 |
| <I/σ(I)> | 13.8 | 0.8 |
| Completeness [%] | 99.9 | |
| Redundancy | 7.5 | |
| CC(1/2) | 0.999 | 0.412 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292.15 | 8% PEG 8000, 0.1 M Bis-Tris pH 6.5, 20% ethylene glycol |
