7LNY
Apo structure of the Histone chaperone ASF1A residues 1-155
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.282, 118.980, 109.506 |
| Unit cell angles | 90.00, 103.45, 90.00 |
Refinement procedure
| Resolution | 106.500 - 2.100 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i32 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.518 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.19) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 106.500 | 106.500 | 2.140 |
| High resolution limit [Å] | 2.100 | 11.500 | 2.100 |
| Rmerge | 0.061 | 0.048 | 0.698 |
| Rmeas | 0.069 | 0.055 | 0.791 |
| Rpim | 0.032 | 0.026 | 0.365 |
| Total number of observations | 2990 | 22481 | |
| Number of reflections | 102510 | 671 | 5048 |
| <I/σ(I)> | 12.8 | 29.3 | 2.1 |
| Completeness [%] | 99.3 | 99.9 | 99.4 |
| Redundancy | 4.4 | 4.5 | 4.5 |
| CC(1/2) | 0.997 | 0.996 | 0.821 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1 ml reservoir of 22% PEG3350 and 6% of Tascimate pH6.0 |
