7LNI

SeMet CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97795
Spacegroup name	P 21 21 21
Unit cell lengths	82.071, 160.985, 231.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.760 - 2.680
R-factor	0.2033
Rwork	0.203
R-free	0.22280
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.423
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.760	2.790
High resolution limit [Å]	2.680	2.680
Rmerge	0.308	2.602
Rpim	0.053	0.513
Number of reflections	164334	16826
<I/σ(I)>	16.1	1.7
Completeness [%]	99.9	99.6
Redundancy	32.9	23.8
CC(1/2)		0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.1 M Tris-HCl pH 7.0, 24.5% PEG 3350, 0.28 M Potassium citrate