7LN8

X-ray radiation damage series on Lysozyme at 277K, crystal structure, dataset 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	277
Detector technology	PIXEL
Collection date	2018-11-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.88557
Spacegroup name	P 43 21 2
Unit cell lengths	77.397, 77.397, 37.205
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.610 - 1.520
R-factor	0.142
Rwork	0.139
R-free	0.20010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7llp
RMSD bond length	0.008
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.700	1.550
High resolution limit [Å]	1.520	1.520
Rmerge	0.102	4.132
Rpim	0.037	1.620
Number of reflections	17920	851
<I/σ(I)>	12.7	0.6
Completeness [%]	99.9	98.1
Redundancy	8.5	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	Lysozyme was dissolved in 0.1 M Sodium Acetate pH 4.6 at 100 mg/ml and 5 microliters of this protein solution was mixed with 5 microliters of 0.1 M Sodium Acetate and 0.6 M Sodium Chloride solution pH 4.6. The 10 microliters drop was equilibrated against 0.7 milliliters of 2.2 M Ammonium sulfate well solution.