7LJJ

Structure of the Exo-alpha-L-galactosidase BpGH29 from Bacteroides plebeius

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-06-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 2 21 21
Unit cell lengths	56.010, 76.430, 132.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.790 - 1.900
R-factor	0.1621
Rwork	0.160
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wvu
RMSD bond length	0.006
RMSD bond angle	0.796
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.081	0.516
Rpim	0.042	0.271
Number of reflections	45650	3045
<I/σ(I)>	15.4	2.9
Completeness [%]	100.0
Redundancy	4.7
CC(1/2)	0.998	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M BIS-TRIS (pH 5.5), 0.2 M (NH4)2SO4, 25% (w/v) PEG 3350