7LJG

Crystal Structure of Lectin from Dioclea altissima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92821
Spacegroup name	P 32 2 1
Unit cell lengths	71.958, 71.958, 161.906
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.318 - 2.210
R-factor	0.2168
Rwork	0.215
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2je9
Data reduction software	xia2
Data scaling software	Aimless (0.5.26)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	161.910	161.910	2.270
High resolution limit [Å]	2.210	9.880	2.210
Rmerge	0.202	0.076	3.425
Number of reflections	25094	352	1842
<I/σ(I)>	8.9
Completeness [%]	100.0	99.9	100
Redundancy	11.6	9.8	9.7
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	100mM Sodium acetate pH4.6, 200mM Ammonium sulphate, 25% PEG4000