7LHN

Crystal structure of Q108K:K40L:T51V:T53C:R58W:T29L:Y19W:Q4A mutant of cellular retinol binding protein II complex with all-trans-retinal after exposure to visible light

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-07-01
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	30.755, 35.773, 64.153
Unit cell angles	86.18, 86.48, 65.22

Refinement procedure

Resolution	29.480 - 2.110
R-factor	0.1811
Rwork	0.172
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qyn
RMSD bond length	0.007
RMSD bond angle	0.871
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.480	2.185
High resolution limit [Å]	2.110	2.110
Rmeas	0.107	0.291
Rpim	0.060	0.163
Number of reflections	12568	1254
<I/σ(I)>	13	4.33
Completeness [%]	88.3	88.93
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	30% PEG 4000, 0.1 M sodium acetate, 0.1 M ammonium acetate , pH 4.6, EVAPORATION, temperature 298K