7LGT
HLA-B*07:02 in complex with 229E-derived coronavirus nucleocapsid peptide N75-83
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.144, 62.854, 63.010 |
| Unit cell angles | 77.15, 77.00, 77.86 |
Refinement procedure
| Resolution | 42.060 - 1.970 |
| R-factor | 0.1916 |
| Rwork | 0.189 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wmn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.12) |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.530 | 44.530 | 2.020 |
| High resolution limit [Å] | 1.970 | 9.030 | 1.970 |
| Rmerge | 0.120 | 0.086 | 0.717 |
| Rmeas | 0.141 | 0.102 | 0.843 |
| Rpim | 0.074 | 0.054 | 0.438 |
| Total number of observations | 204851 | 2124 | 14311 |
| Number of reflections | 56880 | 591 | 3904 |
| <I/σ(I)> | 5.5 | 12.7 | 1.7 |
| Completeness [%] | 98.1 | 98.7 | 96.8 |
| Redundancy | 3.6 | 3.6 | 3.7 |
| CC(1/2) | 0.990 | 0.975 | 0.552 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18% PEG 3350, 0.2 M KI |
