7LGR
Streptococcus mutans Collagen binding Protein CNM - N2 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 48.553, 48.553, 106.424 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.050 - 1.600 |
| Rwork | 0.192 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f6a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.305 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.050 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.120 | 2.175 |
| Rmeas | 0.126 | 2.286 |
| Rpim | 0.039 | 0.699 |
| Number of reflections | 19890 | 961 |
| <I/σ(I)> | 13.5 | 1.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.5 | 10.7 |
| CC(1/2) | 0.999 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 1.5M ammonium sulfate, 100mM sodium acetate, pH 4.5 or 100mM citric acid, pH 3.5 |
