7LGO
Crystal structure of the nucleic acid binding domain (NAB) of Nsp3 from SARS-CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-01-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 76.672, 76.672, 204.146 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.910 - 2.450 |
| R-factor | 0.2636 |
| Rwork | 0.261 |
| R-free | 0.31750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2k87 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.838 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.490 |
| High resolution limit [Å] | 2.440 | 2.450 |
| Rmerge | 0.143 | 1.352 |
| Rpim | 0.040 | 0.386 |
| Number of reflections | 13937 | 666 |
| <I/σ(I)> | 17.7 | 1.36 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 11.4 | 10 |
| CC(1/2) | 1.000 | 0.633 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2 M ammonium sulfate, 2% hexanediol. Cryoprotectant paratone. |
