7LGC
MOAP1 CA-like C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-21 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.034, 34.126, 57.673 |
| Unit cell angles | 97.73, 93.91, 94.64 |
Refinement procedure
| Resolution | 30.160 - 1.850 |
| R-factor | 0.2092 |
| Rwork | 0.207 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Low resolution experimental model |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.757 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.084 | 0.797 |
| Rmeas | 0.093 | 1.000 |
| Rpim | 0.039 | 0.604 |
| Number of reflections | 20575 | 713 |
| <I/σ(I)> | 18.35 | 1.3 |
| Completeness [%] | 94.8 | 65.5 |
| Redundancy | 4.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Tris 20% PEG 3350 3% hexanediol |
