7LG4
Green fluorescent protein from Aequorea macrodactyla - amacGFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | Excillum MetalJet D2+ 70 kV |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-10 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.3418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.089, 47.488, 69.294 |
| Unit cell angles | 90.00, 102.01, 90.00 |
Refinement procedure
| Resolution | 22.600 - 1.810 |
| R-factor | 0.1838 |
| Rwork | 0.182 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2awk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.650 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.600 | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Number of reflections | 19894 | 2932 |
| <I/σ(I)> | 9.36 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.59 | |
| CC(1/2) | 0.996 | 0.624 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | amacGFP (12 mg/mL in 20 mM Tris-HCl buffer, pH 7.5) was crystallized at 21 C in 8% PEG 6K, 3% glycerol, 0.1 M sodium acetate, pH 5.0 supplemented with 5.0% Jeffamine M-600 pH 7.0 according to the Hampton Research Additive Screen protocol |
