7LG0

Human leukocyte antigen B*07:02 in complex with SARS-CoV2 epitope SPRWYFYYL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-09
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9794
Spacegroup name	C 2 2 21
Unit cell lengths	71.723, 155.172, 88.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.950 - 2.300
R-factor	0.2021
Rwork	0.200
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6at5
RMSD bond length	0.005
RMSD bond angle	1.377
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.950	2.340
High resolution limit [Å]	2.296	2.300
Rmerge	0.100
Number of reflections	22281	1096
<I/σ(I)>	8.9	2.2
Completeness [%]	99.6	97.6
Redundancy	6	5.7
CC(1/2)		0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	290	0.1 M HEPES-NaOH, pH 7.5, 2 M ammonium sulfate