7LFO
Protein Tyrosine Phosphatase 1B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-11-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.023, 88.023, 104.494 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.584 - 1.940 |
| R-factor | 0.2025 |
| Rwork | 0.200 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a5j |
| Data reduction software | DIALS (1.12.5-gf5096b3367-release) |
| Data scaling software | xia2 (0.5.769-ga0b6e151-dials-1.12) |
| Phasing software | PHENIX (1.14_3260) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.584 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 35232 | 20873 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11.6 | |
| CC(1/2) | 0.995 | 0.242 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | 140 g/L PEG8000, 100 mM HEPES, 200 mM magnesium acetate, pH 7.5 |
