7LFM
MODEL OF MHC CLASS Ib H2-M3 WITH MOUSE ND1 N-TERMINAL HEPTAPEPTIDE, VAL MUTANT, TRICLINIC CELL, REFINED AT 1.60 ANGSTROMS RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-01-04 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.908 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.743, 65.019, 54.584 |
| Unit cell angles | 98.55, 98.22, 112.36 |
Refinement procedure
| Resolution | 19.090 - 1.600 |
| R-factor | 0.1843 |
| Rwork | 0.184 |
| R-free | 0.21730 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1mhc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.117 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.100 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.450 |
| Number of reflections | 87557 | 4287 |
| <I/σ(I)> | 7.8 | 1.2 |
| Completeness [%] | 84.9 | 46.2 |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 0.1 M Hepes, 20% (w/v) PEG 4000, 30% (v/v) ethylene glycol |
