7LFB

Fab 7D6 bound to ApoL1 NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-09-07
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	94.238, 60.262, 124.216
Unit cell angles	90.00, 111.82, 90.00

Refinement procedure

Resolution	49.628 - 1.913
R-factor	0.1692
Rwork	0.168
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fvd
RMSD bond length	0.006
RMSD bond angle	0.961
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.628	1.930
High resolution limit [Å]	1.890	1.890
Rmerge	0.049	0.425
Rmeas	0.068	0.584
Rpim	0.047	0.339
Number of reflections	51638	3305
<I/σ(I)>	11.4	2.2
Completeness [%]	99.6	99.9
Redundancy	3.3	3.3
CC(1/2)	0.998	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	15%(w/v) PEG 4000, 0.15M Ammonium sulfate, 0.1M MES pH 6